Associate Scientist/Scientist; Stem Cell Biology

Job Location

Seattle

Type of Job

Full Time

Job Description

Pluristyx is seeking an innovative and talented Associate Scientist/Scientist with hands-on knowledge in stem cell biology, induced Pluripotent Stem Cell (iPSC) reprogramming, differentiation, characterization, disease modeling, genome editing, and assay development. This position also requires expertise and experience in cell culturing, molecular biology techniques, microscopy, molecular cloning and gene expression analysis such as real- time polymerase chain reaction (RT-PCR). The candidate will be responsible for completing and assisting with daily tasks associated with PSC-related expansion and processing. Primary duties will also include producing and characterizing iPSC lines, differentiating and characterizing PSC progeny, and manufacturing final product for commercial distribution, including the creation and revision of relevant SOPs, and performing daily tasks in a controlled (possibly cGMP) environment. Candidate will be able to follow established Pluristyx rules, regulations, and safety precautions, documentation practices, and maintain quality standards. The candidate will work closely with internal functional teams and external clients and provide scientific results that support the generation of publications, patents, or regulatory submissions.

Requirements

Education & Experience

  •  PhD in Stem Cell Biology or related discipline;

  • Minimum five (5) years of experience in a stem cell research laboratory with human embryonic stem cell (ESC) and/or induced pluripotent stem cell (iPSC);

  • Demonstrated experience in generation, differentiation, and banking of PSCs and other mammalian cell types;

  •  Experience in Current Good Manufacturing Practices environment and flow cytometry preferred.


Duties & Daily Tasks

  • Reprogramming human somatic cell lines into induced pluripotent stem cells (iPSCs);

  • Expanding ESCs and iPSCs in culture and banking for research use;

  • Differentiating iPSCs into leukocytes, cardiomyocytes (CMs), neurons, beta islet cells and other cell types;

  • Development of disease modeling assays through assessment of physiologically relevant phenotypes such as contractility, calcium imaging, bioenergetics profiling, and electrophysiology;

  • Development of disease modeling assays through comparative experiments of iPSC derived progeny with analogous human tissue; Genome editing of iPSC lines for mutation introduction and correction;

  • Perform facilities maintenance as needed, including ordering of supplies, materials, and equipment;

  • Follow, create, and update SOPs as needed;

  • Perform daily tasks in a controlled (possibly cGMP) environment;

  • Assist in training new and existing employees on the cell expansion manufacturing process;

  • Assist in providing positive and constructive individual feedback and building teamwork;

  • Ability to lift 25lbs. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions

  • Other responsibilities as assigned.


Other Skills & Abilities

  • Ability to interact with subject matter experts in process and analytical development, stem cell and iPSC culture, industry and business representatives;

  • Ability to clearly write and present data and reports;

  • Strong written and oral communication skills;

  • Ability to make oral presentations to clients/collaborators;

  • Good organizational skills and detail-oriented;

  • Ability to work independently and in a team environment;

  • Relevant computer skills.


Salary

Salary will be commensurate with education, relevant work experience and other qualifications.

To apply please email your CV to careers@pluristyx.com

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